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4-channel imaging with long Stokes shift fluorophores

Current Protocols

This article describes multiplexed labeling and imaging of four target antigens through the use of a long-Stokes-shift fluorophore in tandem with three conventional fluorophores.

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SEQUIN code and sample datasets

GitHub repository

Synaptic Evaluation and QUantification by Imaging of Nanostructure

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SEQUIN Protocol

STAR Protocols

SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse