Tools/Datasets

4-channel imaging with long Stokes shift fluorophores
Current Protocols
This article describes multiplexed labeling and imaging of four target antigens through the use of a long-Stokes-shift fluorophore in tandem with three conventional fluorophores.

SEQUIN code and sample datasets
GitHub repository
Synaptic Evaluation and QUantification by Imaging of Nanostructure

SEQUIN Protocol
STAR Protocols
SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse