Tools and datasets
SEQUIN Protocol
STAR Protocols
SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse
SEQUIN code and sample datasets
GitHub repository
Synaptic Evaluation and QUantification by Imaging of Nanostructure
4-channel imaging with long Stokes shift fluorophores
Current Protocols
This article describes multiplexed labeling and imaging of four target antigens through the use of a long-Stokes-shift fluorophore in tandem with three conventional fluorophores.
Featured publications
SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury
September 1, 2020
Sauerbeck AD, Gangolli M, Reitz SJ, Salyards MH, Kim SH, Hemingway C, Gratuze M, Makkapati T, Kerschensteiner M, Holtzman DM, Brody DL, Kummer TT. SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury. Neuron. 2020 Jul 22;107(2):257-273.e5. doi: 10.1016/j.neuron.2020.04.012. Epub 2020 May 8. PMID: 32392471; PMCID: PMC7381374.
Recent publications
Enhanced Multiplexing of Immunofluorescence Microscopy Using a Long-Stokes-Shift Fluorophore
August 1, 2021
Reitz SJ, Sauerbeck AD, Kummer TT. Enhanced Multiplexing of Immunofluorescence Microscopy Using a Long-Stokes-Shift Fluorophore. Curr Protoc. 2021 Aug;1(8):e214. doi: 10.1002/cpz1.214. PMID: 34387945; PMCID: PMC8375615.
Neuronal VCP loss of function recapitulates FTLD-TDP pathology
July 20, 2021
Wani A, Zhu J, Ulrich JD, Eteleeb A, Sauerbeck AD, Reitz SJ, Arhzaouy K, Ikenaga C, Yuede CM, Pittman SK, Wang F, Li S, Benitez BA, Cruchaga C, Kummer TT, Harari O, Chou TF, Schröder R, Clemen CS, Weihl CC. Neuronal VCP loss of function recapitulates FTLD-TDP pathology. Cell Rep. 2021 Jul 20;36(3):109399. doi: 10.1016/j.celrep.2021.109399. PMID: 34289347; PMCID: PMC8383344.
SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse
January 13, 2021
Reitz SJ, Sauerbeck AD, Kummer TT. SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse. STAR Protoc. 2021 Jan 13;2(1):100268. doi: 10.1016/j.xpro.2020.100268. PMID: 33490984; PMCID: PMC7811167.
Impact of TREM2R47H variant on tau pathology-induced gliosis and neurodegeneration
September 1, 2020
Gratuze M, Leyns CE, Sauerbeck AD, St-Pierre MK, Xiong M, Kim N, Serrano JR, Tremblay MÈ, Kummer TT, Colonna M, Ulrich JD, Holtzman DM. Impact of TREM2R47H variant on tau pathology-induced gliosis and neurodegeneration. J Clin Invest. 2020 Sep 1;130(9):4954-4968. doi: 10.1172/JCI138179. PMID: 32544086; PMCID: PMC7456230.