Tools & datasets
SEQUIN Protocol
STAR Protocols
SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse
SEQUIN code and sample datasets
GitHub repository
Synaptic Evaluation and QUantification by Imaging of Nanostructure
4-channel imaging with long Stokes shift fluorophores
Current Protocols
This article describes multiplexed labeling and imaging of four target antigens through the use of a long-Stokes-shift fluorophore in tandem with three conventional fluorophores.
Featured publications
SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury
September 1, 2020
Sauerbeck AD, Gangolli M, Reitz SJ, Salyards MH, Kim SH, Hemingway C, Gratuze M, Makkapati T, Kerschensteiner M, Holtzman DM, Brody DL, Kummer TT. SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury. Neuron. 2020 Jul 22;107(2):257-273.e5. doi: 10.1016/j.neuron.2020.04.012. Epub 2020 May 8. PMID: 32392471; PMCID: PMC7381374.
Recent publications
- Auricular vagus nerve stimulation for mitigation of inflammation and vasospasm in subarachnoid hemorrhage: a single-institution randomized controlled trialHuguenard, A. L., Tan, G., Rivet, D. J., Gao, F., Johnson, G. W., Adamek, M., Coxon, A. T., Kummer, T. T., Osbun, J. W., Vellimana, A. K., Limbrick, D., Zipfel, G. J., Brunner, P. & Leuthardt, E. C., Jun 2025, In: Journal of neurosurgery. 142, 6, p. 1720-1731 12 p.Research output: Contribution to journal › Article › peer-review
- Brain injury drives optic glioma formation through neuron-glia signalingChatterjee, J., Koleske, J. P., Chao, A., Sauerbeck, A. D., Chen, J. K., Qi, X., Ouyang, M., Boggs, L. G., Idate, R., Marco Y Marquez, L. I., Kummer, T. T. & Gutmann, D. H., Dec 2024, In: Acta Neuropathologica Communications. 12, 1Research output: Contribution to journal › Article › peer-review
- Non-invasive Auricular Vagus nerve stimulation for Subarachnoid Hemorrhage (NAVSaH): Protocol for a prospective, triple-blinded, randomized controlled trialHuguenard, A., Tan, G., Johnson, G., Adamek, M., Coxon, A., Kummer, T., Osbun, J., Vellimana, A., Limbrick, D., Zipfel, G., Brunner, P. & Leuthardt, E., Aug 1 2024, In: PloS one. 19, 8, e0301154.Research output: Contribution to journal › Article › peer-review
- Diffusion basis spectrum imaging detects subclinical traumatic optic neuropathy in a closed-head impact mouse model of traumatic brain injuryYang, H. C., Lavadi, R. S., Sauerbeck, A. D., Wallendorf, M., Kummer, T. T., Song, S. K. & Lin, T. H., 2023, In: Frontiers in Neurology. 14, 1269817.Research output: Contribution to journal › Article › peer-review
- Recombinant human erythropoietin induces neuroprotection, activates MAPK/CREB pathway, and rescues fear memory after traumatic brain injury with delayed hypoxemia in miceCelorrio, M., Rhodes, J., Shumilov, K., Moritz, J., Xiao, S., Anabayan, I., Sauerbeck, A., Kummer, T. & Friess, S., Nov 15 2022, In: Brain Research. 1795, 148074.Research output: Contribution to journal › Article › peer-review
- Sex Differences in the Role of CNIH3 on Spatial Memory and Synaptic PlasticityFrye, H. E., Izumi, Y., Harris, A. N., Williams, S. B., Trousdale, C. R., Sun, M. Y., Sauerbeck, A. D., Kummer, T. T., Mennerick, S., Zorumski, C. F., Nelson, E. C., Dougherty, J. D. & Morón, J. A., Dec 1 2021, In: Biological Psychiatry. 90, 11, p. 766-780 15 p.Research output: Contribution to journal › Article › peer-review
- Unbiased high-content screening reveals Aβ- And tau-independent synaptotoxic activities in human brain homogenates from Alzheimer's patients and high-pathology controlsJiang, H., Esparza, T. J., Kummer, T. T. & Brody, D. L., Nov 2021, In: PloS one. 16, 11 November, e0259335.Research output: Contribution to journal › Article › peer-review
- Enhanced Multiplexing of Immunofluorescence Microscopy Using a Long-Stokes-Shift FluorophoreReitz, S. J., Sauerbeck, A. D. & Kummer, T. T., Aug 2021, In: Current Protocols. 1, 8, e214.Research output: Contribution to journal › Article › peer-review
- Neuronal VCP loss of function recapitulates FTLD-TDP pathologyWani, A., Zhu, J., Ulrich, J. D., Eteleeb, A., Sauerbeck, A. D., Reitz, S. J., Arhzaouy, K., Ikenaga, C., Yuede, C. M., Pittman, S. K., Wang, F., Li, S., Benitez, B. A., Cruchaga, C., Kummer, T. T., Harari, O., Chou, T. F., Schröder, R., Clemen, C. S. & Weihl, C. C., Jul 20 2021, In: Cell Reports. 36, 3, 109399.Research output: Contribution to journal › Article › peer-review
- Localizing focal brain injury via EEG spectral varianceKhanmohammadi, S., Laurido-Soto, O., Eisenman, L. N., Kummer, T. T. & Ching, S. N., Jul 2021, In: Biomedical Signal Processing and Control. 68, 102746.Research output: Contribution to journal › Article › peer-review