Tools & datasets

SEQUIN Protocol

STAR Protocols

SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse

SEQUIN code and sample datasets

GitHub repository

Synaptic Evaluation and QUantification by Imaging of Nanostructure

4-channel imaging with long Stokes shift fluorophores

Current Protocols

This article describes multiplexed labeling and imaging of four target antigens through the use of a long-Stokes-shift fluorophore in tandem with three conventional fluorophores.

Featured publications

SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury

September 1, 2020

Sauerbeck AD, Gangolli M, Reitz SJ, Salyards MH, Kim SH, Hemingway C, Gratuze M, Makkapati T, Kerschensteiner M, Holtzman DM, Brody DL, Kummer TT. SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury. Neuron. 2020 Jul 22;107(2):257-273.e5. doi: 10.1016/j.neuron.2020.04.012. Epub 2020 May 8. PMID: 32392471; PMCID: PMC7381374.

Recent publications